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... directed to the proper target sequence. They succeeded in labeling two different pairs of chromosomes...

...on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia (that would...

...diverse species. These results further highlight the need for in situ genetic tools for microbiome studies...

...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can result...

...foremost, be mindful of your extractions- if you need really clean RNA, it’s important to make sure that...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

...detect RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein...

... DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique...

...variety of useful resources and protocols! We invite feedback from all members of the research community to ...

...deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based delivery...

... the insertion of the GFP tag precisely where we need it, in this example, at the C-terminus of the gene...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

...’re just starting out with prime editing, you’ll need to design and optimize a pegRNA for your particular...

...direct transfer from one cell to another). Bacteria need special fertility genes to be capable of conjugation...

... a Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. ...

... single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space for multiplexing...

...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...

...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

...Another potential limitation of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline...