We narrowed to 699 results for: eed

1. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...foremost, be mindful of your extractions- if you need really clean RNA, it’s important to make sure that...

2. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu, B., et al. Cas9...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...detect RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein...

4. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ... DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique...

5. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ... directed to the proper target sequence. They succeeded in labeling two different pairs of chromosomes...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...variety of useful resources and protocols! We invite feedback from all members of the research community to ...

7. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can result...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

9. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ... a Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. ...

11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...

12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...Another potential limitation of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline...

14. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...’re just starting out with prime editing, you’ll need to design and optimize a pegRNA for your particular...

15. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...direct transfer from one cell to another). Bacteria need special fertility genes to be capable of conjugation...

16. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ... single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space for multiplexing...

17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ... the insertion of the GFP tag precisely where we need it, in this example, at the C-terminus of the gene...

18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based delivery...

19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...

20. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...