We narrowed to 687 results for: eng

...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...