...much time you have to spend cloning before you can start your experiment? What about all the reagents you...you did everything correctly the first time – no mistakes, no repeats! How long does it take in total? While...occurred. Here at Addgene, the consumables cost per stab has increased 50% since 2019, while overall inflation...

...familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas ...Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s...primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a cleavage...

... miniature CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (...also created a miniature yet active Cas system starting with the same Un1Cas12f1 (Cas14). They noted that...and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del...

.... coli and yeast. Because this approach uses standardized parts, it allows for greater reproducibility...CO2 into water and electrical energy generation. Alistair McCormick’s lab recently developed the CyanoGate...muscle and epithelial cells, as well as in different stages of development, ensuring the breadth and versatility...

... our lab used jargon that required context to understand, I came to realize that there were a lot of words...a list of frequently used words like this when starting rotations or my postdoc would have simplified ...Feeling like people aren’t listening to you or understanding you can be incredibly frustrating. Clarifying...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...of a single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed...GFP regulation to other types of proteins. They started with another biological workhorse, Cre recombinase...

...for a successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who...in this group have scientific backgrounds. For a stated purpose of transitioning a scientist from academia... to Life. She runs great tweet chats, has an outstanding blog and, while her PhD is in History, I think...

...into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived...Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...

...coffee grounds could be economically repurposed as starting material for the bioproduction of therapeutic ...substrate, inducing a conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring...dark fluorescent proteins, it has good signal stability allowing for minimized basal FRET variability,...

... the F1 generation, your breeding scheme should start with parental mice that are each homozygous for ...chromosomes in the parental generation, they are likely to stay on separate chromosomes in future generations as...homozygous for both mutations. The shorter the distance between the two loci of interest on the chromosome...

... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...elegans. To study the role of 3’UTRs, the Arizona State University (ASU) Biodesign institute developed a...opportunities to develop computational methods to better understand animal development (Kyoda et al., 2012).  C. ...

...cell cycle attempt to understand development, regeneration, and certain disease states. However, it’s difficult... each tool expanding our ability to study and understand the complex interworking of the 86 billion neurons...

..., even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every supervisor... details of your work. Describe the problem by stating the facts and explain how it is interfering with...is miscommunication. For example, you might misunderstand your supervisor’s suggestions and take your ...

...similar regions. This combination of chewing up one stand and cutting the template strand causes large deletions... to studying Cas3 and its Cascade partners to understand it and use it to its full potential. Hopefully...Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R....

...degradation of the tagged protein. Degrons are destabilizing domains which will be identified by various ...protein must be expressed (inducibly, ectopically, stably… it’s your call). The TIR1 F-box protein will associate.... If your project also involves imaging at some stage, then choosing a HaloTag could pay dividends down...

...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...cells did not seem to form discrete colonies but constantly migrated away from each other. In our experience...

...glycosylation and acetylation to create functional, stable proteins. Protein expression refers to the second...can express proteins both transiently or through stable cell lines. Both methods produce high protein yields...adapted to fermentation allowing for large-scale, stable production of proteins. In general yeast expression...

...questions. Practice your presentation for both instances. If your poster material is nearing publication... this is being used, it’s best to turn to an established social media platform. During the conference ...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...

...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...definitive, but visual identification is a good place to start before taking on more time consuming and expensive...use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used codon optimization programs...

...immersed in an industry environment, have a better understanding of the fast, deadline-driven pace of biotech...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at...