We narrowed to 855 results for: NTS

...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples of factors...HSF-1), which subsequently binds to heat shock elements in the promoter, thereby activating transcription...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...

.....we’re excited to help scientists share their reagents with the scientific community.   (click to enlarge...it ideal for CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through... Zhang lab has shared over 40,000 CRISPR-Cas9 components over the years on their own or through Addgene...used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC...Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse transcription, and integration...our depositors and requestors for making their reagents available and for supporting scientific sharing...

...diseases. They do that by studying patients to uncover new gene variants associated with conditions of interest...interest, then studying those variants in model systems: either human cells or mice. In a recent issue...implicated in disease or, in cases where particular variants or mutations have been reported in people, we .... You could use the kit to insert all of those variants into stem cells with the same genetic background...make induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have...

...used together in the same experiment because the reagents used to detect the presence of an antibody depends...applications for antibodies You can use antibodies for experiments in a variety of fields including neuroscience...lysates, whole cells, or tissue samples. Many experiments that use antibodies use a primary antibody, which...buffer. Then, the purified protein can be used in experiments to compare the relative amount of protein present...such as Western blots or ELISAs, or for other experiments to study protein-protein interactions. Flow cytometry...2016) Aptamers, antibody scFv, and antibody Fab’ fragments: An overview and comparison of three of the most...most versatile biosensor biorecognition elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org...

...experiment work? What are the various tools and components necessary to run it? And, most importantly, what...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot speak...any optogenetic experiment. There are numerous variants for the different types of opsins available and...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...in which you monitor expression at various time points (e.g. every 1-2 weeks over 8 weeks) in order to...science considerations for your optogenetics experiments. Stay tuned!  Many thanks to our guest blogger...

...ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression in a particular...genome editing, optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...vectors with multiple genes packaged in them and use elements such as IRES or 2A to co-express them from one...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide ...

...ChRger. In both experiments, neurons expressing ChRgers produced stronger photocurrents at low light intensity...invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly ...properties best suited for less-invasive optogenetics experiments. The sequences in this library were designed ...in vivo minimally invasive optogenetics. Both experiments delivered ChRgers by intravenous injection of...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...

...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme...and is particularly convenient for targeting experiments requiring single strand DNA generation, such ... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast... the other Cas family members make for useful implements in the scientist toolbox. If your genome engineering.... Choosing the right Cas can really put your experiments Nsync.   References and resources   References...

...guide you through the basics of CombiGEM-CRISPR experiments. CombiGEM-CRISPR: The marriage of two simple ...interrogating their functions. The continual advancements in gRNA design necessary to achieve maximal ...highly flexible and can accommodate any genetic elements of interest. It can thus be tailored to address... the combinatorial expression of other genetic elements including transcription factors (Cheng et al.,...et al., 2015). Starting your CombiGEM-CRISPR experiments The first thing you will need is to get the list...should be verified by NGS before beginning your experiments. Figure 2: Strategy for assembling barcoded...

...where to look for a coach. Many (most?) graduate students and postdocs may feel they have limited funds ...references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have loads of success...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...through your PhD? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...