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We narrowed to 843 results for: NTS

Showing: 321 - 330 of 843 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...where to look for a coach. Many (most?) graduate students and postdocs may feel they have limited funds ...references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have loads of success...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...through your PhD? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...questions. Story telling shares many of the same elements as a STAR answer: a setting, a conflict, and a...which the story demonstrates I possess.   Bullet points on resumes are like cliff notes, so I use these.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...professional  First impressions matter, so make sure yours counts. Dress professionally but still like yourself. ...experience less stressful. First, you can make arrangements with those you live with to have a quiet place...talk. Some video conferencing software allows participants to “raise” their hand to ask a question or type...

  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ... for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally from E.coli,...modified ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round...x-d.  This resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency...PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target many additional locations in...cells and return the corrected cells back to the patients. For example, defects in hemoglobin (𝛽-globin...continuous expression of fetal hemoglobin even in adult patients. In order to analyze the clinical use of ABE8 ...

  4. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...share, remix, store, and track these essential components of the scientific toolbox. What are Recombinant...already begun creating plasmids encoding affinity reagents. These include conventional heavy and light chain...nanobodies, ScFVs) and non-antibody based affinity reagents (e.g., monobodies, DARPins) (Helma et al., 2015...what antibody they are using and any validation experiments performed will be useful in perpetuity.  Second...CRISPR community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding... AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038...

  5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...first split into two non-functional fragments. When these two fragments are near each other, they combine...non-functional fragments: N-terminal BirA* (N-BirA*) and C-terminal BiraA* (C-BirA*). These two fragments are fused...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...When  proteins Y and Z interact, the two BirA* fragments complement each other to form a functional BirA...

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...can be used to induce site-specific recombination events, and that the orientation of the flanking loxP ...-the FLEx switch--which utilizes recombination elements to turn off expression of one gene, while simultaneously...target sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds... the DNA fragment, any additional recombination events are impossible even in the presence of Cre recombinase...wide variety of in vivo conditional gene rescue experiments, as well as for improvement of optogenetic adeno-associated...Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...prokaryotic or eukaryotic, based on the functional elements they contain. Plasmid DNA in both prokaryotic ...typically occur directly after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal...Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0...terminators to reduce unwanted translation of downstream elements. A high affinity terminator may be desired for...non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use weaker...Gil A, Proudfoot NJ. Position-dependent sequence elements downstream of AAUAAA are required for efficient...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...was the outdoor market where one can shop for components of traditional Chinese medicine. There were hundreds...flora or fauna. I tried to find out what certain ingredients might be used for (like what would one take dried...genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when small RNA...tRNAs and tRNA-derived RNAs. Stay tuned for big developments in this field. Nobel Laureates and the ever ...furiosus, a hyperthermophile that exists in sea vents at the boiling point of water. Dr. Terns gave a ...Yat-sen University presented two new technical developments. Using the Tet-on system, his lab has created...

  9. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. ...understand this complexity using existing C. elegans mutants. For instance, we use a mutant known as CX51 that...we have the capability to study many different mutants because the Caenorhabditis Genetics Center (CGC...model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour, and ultimately ...sequencing again - it’s too inefficient and the reagents are too expensive to make it practical - but we...

  10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...human body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak... of Queensland) to observe membrane trafficking events in vivo. Locate your protein and see who it is ...structures. In order to interpret colocalization experiments in a scientific and meaningful way it is important...are not without limitations and well-executed experiments require some consideration of FP properties. ...developed FPs optimized for diverse cellular environments. Since colocalization relies upon the detection... for fluorescently tagged viral and bacterial components. For example, the Rainbow Vectors from the Mariette...
Showing: 321 - 330 of 843 results