We narrowed to 599 results for: REV;

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ... 2. See which ESCs die or differentiate Step 3 reveals which NANOG-related genes are critical to stem ...using Bedtools getFasta. These regions and their reverse complements were parsed for spCas9 PAM sites (NGG...

2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...take months of time for your manuscript to get reviewed and edited prior to publication, and this will...hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE allows scientists to join...

3. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition...

4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  perhaps you... (Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions...

5. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...

6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...pCXLE episomal vector toolkit It has been shown previously that suppression of TP53 enhances iPSC generation...peripheral blood, a huge improvement from the previously reported efforts. The pCXLE episomal system thus...

7. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...

8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...require a PAM, and RNA targeting is potentially reversible since there is no genomic edit. Some Cas13 enzymes...KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

9. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech...RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher...

10. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...the questions.  After the code is written, it is reviewed by at least one other developer. This is another...point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into the mind of...