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We narrowed to 895 results for: Ide

Showing: 641 - 660 of 895 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools...adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic applications Vaccine...popular nucleic acid-based vaccines. These vectors provide several advantages over traditional vaccines, including...and cell-specific targeting features make them an ideal delivery system for human patients. Multiple viral-based...

  2. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...relationships than at scientific conferences. Conferences provide the opportunity to meet people who are interested...larger your profile in the community Job hunting outside of academia is all driven by personal connections...you are spending on a conference? One thing to consider is the size of the conference. Large conferences...scientists at your level of training is always a good idea. Make contact with other attendees in advance. Join...

  3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...Visit our viral vectors guide Find our chemogenetics and optogenetics science guides ...tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain is a tricky organ... Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin engineering enables minimally ...

  4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ... more confidence in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating... replication are initiated before the cell has divided. This means that genes located near the origin ...engineering, the expression of enzymes in a pathway will ideally be optimized to promote generation of the desired...

  5. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ... the Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...Tagging relevant proteins with a degron tag can provide inducible protein degradation. These systems work...

  6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...Addgene Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts...single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed in ...these fusions retained the ability to bind GFP. The idea of using GFP as a scaffold suddenly seemed very ...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...translational termination features with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette...knockout – conversely, putting the loxP sites on either side of a gene (called “floxing”, for “flanked by loxP...experimental constraints. This has been used for a wide range of purposes, including activating an oncogene...switch recombination at individual switch regions evidenced through Cre–loxP–mediated gene targeting. Gu, ...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ... cells that make up the human body and G-baToN provides versibility in tracking and modulating the interactions...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM... viral aliquots from our plasmid collection to provide ready-to-use viral preps. Here are some of the ...

  9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...CRISPR Background with Our Guide Pages Other Links Check out Pawluk et al's Video Abstract Read up on All...off-target edits of base editors, which convert a nucleotide base pair to another base pair at a specific .../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...respectively. Primary cell type Gene targeted by guide Editing efficiency hiPSCs  EMX1  67% Mouse BM...in cells electroporated with RNPs using the same guide. Nanoblades also had lower off-target effects when...insertion of a DNA sequence ranging from a single nucleotide change to large insertions like the addition ...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...measure the tension in the helical structure of spider silk, or the activity of a protein in a synapse... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...As the choice of fluorophore pairs is a key consideration for a FRET experiment, the possibilities can...PMCID: PMC3810607. 2. Bajar, Bryce T., et al. "A Guide to Fluorescent Protein FRET Pairs." Sensors 16.9...

  12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... are considered large deposits and are exceptions to our standard workflow.  Table 1: Guidelines for the...Plasmids with Addgene” blog post for a thorough guide to our online data entry process. Figure 1: Begin...application of the plasmid and will help a scientist outside your field understand if this plasmid will work...

  13. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...rescued by zygote co-injection of Cas9 and a single-guide RNA targeting the mutant allele. When all was said...hATTR) hATTR is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...CRISPR therapies have made significant clinical strides since we first wrote this blog post. The future...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...bigger movement in a variety of research sectors to provide open source technologies and spaces where interested...One of the aspects of this project that makes it ideal for makerspaces is the data is freely available,...projects). This allows the project to be open to a wider group of people. This is a perfect starter project...equipment becomes obsolete amazingly fast, but with a wide range of skills among our members we’ve managed ...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...powerful than it is. The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9...Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide unwinding steps. Molecular Cell, 70(3), 385-394...Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3. Science, 361...CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including...certain in vitro and in vivo applications, you can consider using unpurified AAV from the supernatant (Goodwin... in precision and variability, qPCR is the more widely used method due to the accessibility of instruments...101 series!  This post was originally written by Didem Goz Ayturk and updated by Ina Ersing in August 2023...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...of contamination for your experiments, the bright side is that you also have the power to take the added...working with so that cross-contamination can be identified. Even if some mammalian cells lines have similar...check under the light microscope will help you identify bacterial and yeast contaminants. Set up a routine...will help you avoid cross-contamination through accidental mixing and will also help you keep good records...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...crystallographer working with Jane Richardson, provided an initial solution to this problem with the development...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...allow the complementary base pairing between the guide RNA and the target DNA strand, and then activate...User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...plasmids to produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep) and capsid (cap) genes...primarily found in the culture media, you should consider performing PEG precipitation. Instead of sonicating... details. Pro tips Watch this AAV purification video! It didn’t exist when I did my first iodixanol gradient... gradient purification, but I wish it had. The video gives a brief overview of how iodixanol purifications...

  20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...wanted to expand the toolkit. We have included a wider range of terminators than are commonly used as we...rusts and we are collecting sequences that will provide a similar resource for those involved in monocot... really and we’ll be producing an instructional video soon to make it easier for people to use the Golden...think plant biology is massively underfunded considering how important it is. I would really like to reach...
Showing: 641 - 660 of 895 results