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...quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer Agreement...quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after all, they...unprocessed agreements slow down your research; provide specific examples if possible Tell them about the...exchange of intellectual property (as inventions, ideas, plasmids) among universities and other research...

...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...dynamic process into a static measurement and it provides limited information about what materials or organelles...autophagic activity. The aim of this post is to provide an overview of four autophagy biosensors currently...protein plasmids and resources Fluorescent protein guide: Biosensors ...

...attention and enjoy anecdotal forms of information — identifying with a character, building emotional investment... do and should tell stories all the time. They provide accounts of their research struggles in papers.... a weekly lab meeting as you’ll need to for an outsider. And you won’t need to dive as deep into your ...preprint servers, share your email address far and wide, keep your website up to date, invite people to ...

...explaining a technical piece of research to others outside that specialty, who may be other scientists, or...journalists, or policy makers. The term editor covers a wide variety of careers and jobs. At Science, which is...financially critical. Those who work on the publishing side of science have professional meetings, societies...in the science communications world, you might consider an internship or a short program to build skills...

...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially ...distribution of cell lines from repositories on opposite sides of the world shows that science is truly a global...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ...15562-9 Subbarao K, Roberts A (2006) Is there an ideal animal model for SARS? Trends in Microbiology 14...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing tool. However, Cas9’s endonuclease...when studying neuronal activity. The researchers identified MerMAIDS from their analysis of different publicly...comprised of thousands of loops. These loops not only provide a 3-D structure for packing genomic DNA into nuclei...

...fusion protein, etc...).  There are many online guides – including an excellent series of posts by Joachim... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary...for, the Table of Fluorescent Protein Properties provides a master list of all proteins in the database,...try out the site and contact us if you have any ideas or requests.  Or, if you have experience in Python...

...performed with embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing...cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat...circulation of following FAP-TAP light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages...Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other...

...that we could discuss our projects and ideas every day and decide on the best way to structure roles and...-group meetings to discuss day-to-day progress, ideas, and ways to improve the lab. “An effective working...including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and New Faculty...

...license. Better performance = better data In side-by-side tests with popular red and yellow GECIs, OCaMP... while red indicators jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently...red.  However, current red and green GECIs aren’t ideal for two-photon imaging with newer lasers in the ...

...addgene.org Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...to upregulation or downregulation are easier to identify by comparing peak positions to the base expression...2. Quantification using MFI makes it easier to identify and study these subtle shifts. Additionally, you...

...lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools...adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic applications Vaccine...popular nucleic acid-based vaccines. These vectors provide several advantages over traditional vaccines, including...and cell-specific targeting features make them an ideal delivery system for human patients. Multiple viral-based...

...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works...up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...ll need to ensure you have a bead-only control alongside your input control. Elute the DNA after washing...you generate your data. However, it may be a good idea to add "patience and an extra-methodical approach...

...this work? Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on ...deaminase, TadA-7.10 (Gaudelli et al., 2017). The idea was to evolve a TadA-7.10 version with a faster ...enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold faster than TadA... base editors for your research Read our CRISPR guide Find plasmids for your research ...

...researchers.There is both theoretical and empirical evidence (in psychology, cancer biology, pre-clinical life...) do you really appreciate how few details are provided in most published papers. The precise timing, ...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity in study...

...relationships than at scientific conferences. Conferences provide the opportunity to meet people who are interested...larger your profile in the community Job hunting outside of academia is all driven by personal connections...you are spending on a conference? One thing to consider is the size of the conference. Large conferences...scientists at your level of training is always a good idea. Make contact with other attendees in advance. Join...

... the Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...Tagging relevant proteins with a degron tag can provide inducible protein degradation. These systems work...

...Visit our viral vectors guide Find our chemogenetics and optogenetics science guides ...tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain is a tricky organ... Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin engineering enables minimally ...

... more confidence in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating... replication are initiated before the cell has divided. This means that genes located near the origin ...engineering, the expression of enzymes in a pathway will ideally be optimized to promote generation of the desired...

...Addgene Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts...single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed in ...these fusions retained the ability to bind GFP. The idea of using GFP as a scaffold suddenly seemed very ...