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We narrowed to 839 results for: LEA

Showing: 641 - 660 of 839 results

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  6. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  7. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  9. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  10. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb...

  11. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  13. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  14. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...reprogramming, and beyond. For further details, please check out Wong et al 2016 and Wong et al 2015. ...PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA Read How ...

  19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...easier. Advantages and other possibilities It’s clear that Cre-DOG and T-DOGGs open up many new possibilities...

  20. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...
Showing: 641 - 660 of 839 results