We narrowed to 848 results for: LEA
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TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....Plasmids 101 Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR...
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Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m.... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style. ... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m.... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....scientists? Curiosity? Passion? A love of learning? No. Or at least not entirely. What truly unites us all... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...blog posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research Learn about our viral service ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m.... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....Symposium on Cancer, Inflammation and Immunity and learn more about “... recent findings in basic immunology... autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions as one of... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al, 2010). An explanation...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....critical roles in nerve and muscle function, hormone release, and cell signaling throughout the body. Because...Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR). ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....consider them worth the trouble. Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....selection markers. The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...