We narrowed to 711 results for: Met
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TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592...
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Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....By being creative, thoughtful, and open to new methods, you’ll be able to find strategies and systems ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m.... your deck. If someone asks a question about something they did not understand about your presentation... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m.... they remember the moments when they discover something new for themselves and when they effectively contribute... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....forward. The viral vector project team develops methods, performs extensive quality control, generates ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m.... of unmodified proteins. Affinity purification methods typically require a protein to be tagged, which... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in the SynBio ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....Prior to Tufts Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing insurance, finance... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....efficiency of TurboID and miniTurbo makes PL a viable method for proteomics in model organisms in which it was... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....equipment that still works. “We use functional spectrometers from the early 1990s that were free and free... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m.... or who your new contact will connect you to. Sometimes, it is not who you know but who knows about you... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....from Cas9 and almost all other Cas enzymes is its method of DNA cleavage. Instead of cleaving DNA at a defined... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....Protein A or Protein G purification is one common method. These proteins bind the IgG heavy chain. Running... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... use of these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens HRP (P) Tyramide signal... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems for high-throughput...