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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 850 results for: NTS

Showing: 641 - 660 of 850 results

  2. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in our repository ...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...distance! To the authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a...

  4. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not...bench research. I am always surprised at how many applicants ignore this in their cover letters. It is also...

  5. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...Individual Development Plans (IDPs) for the graduate students and postdocs in their labs. Dr. Richard Roberts... industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

  6. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...set by the properties of hemoglobin and melanin pigments, which absorb the majority of light below 650 ... iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals. The approach ...

  8. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...compiles a list of hundreds of different jobs for students to apply to, and after a grueling two months of...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

  9. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Like promoter traps, enhancer traps lack enhancer elements and require the trapping of endogenous enhancers...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

  10. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited...literature, or to decide which line is best for your experiments. You can also use the Cellosaurus STR similarity...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...understanding of a GPCR mediated response.  Acknowledgements Special thanks to Jeff DiBerto, a graduate...fluorescent protein plasmids Find biosensors for your experiments ...

  12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...

  13. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...powerhouse depositors who have generously shared their reagents freely with the research community. Sharing is...logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors to...

  15. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    .... To help you find the vector that fits your experiments, I've described below some of the most frequently...backbone. Or just ask –  leave a message in the comments below and an Addgenie will help you find what ...

  16. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen...

  17. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...

  18. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ...

  19. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated...

  20. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...
Showing: 641 - 660 of 850 results