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Showing: 641 - 660 of 690 results
  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...
  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...
  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...
  4. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...
  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...
  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence...
  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...This is similar to the proposed mechanism for engineered pegRNAs (epegRNAs), which add a structural motif...
  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...
  9. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from...
  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...
Showing: 641 - 660 of 690 results