We narrowed to 818 results for: lars
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TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....deposited from Di Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here...page Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids ...
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Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments. References... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m.... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...Protoctols for Your Research Catch Up On Your Molecular Biology Basics ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology. https://doi.org/10.1002/jcp.30064... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....would take years and millions, if not billions, of dollars, but by approaching it from a new direction, we...Science Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... dialogue and community among participants, clarification - new and useful information, and invigoration...meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 minutes weekly... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....post-transcriptional gene expression across the majority of cellular pathways. Despite several years of research, the...pushed the field further with the creation of the largest miRNA sensor collection reported to date, containing... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m.... through my work on the audit committee. I’m particularly curious to see how that progresses in 2019 and...Before you can go off and do great things in a particular area or profession, you need to make sure you... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....Guide" from Nature Jobs Blog While Twitter is a popular platform for scientists, it’s not the only tool...statistics on what percent of scientists and engineers regularly use the platform, why scientists are using the... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....systems, methyltransferases, methylate DNA at particular sequences to prevent them from being degraded...Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...