We narrowed to 811 results for: lars
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TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes...
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Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology. https://doi.org/10.1002/jcp.30064... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m.... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...Protoctols for Your Research Catch Up On Your Molecular Biology Basics ... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... dialogue and community among participants, clarification - new and useful information, and invigoration...meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 minutes weekly... -
Fluorescent Proteins 101: Luciferase
TypeBlog PostPublishedJune 24, 2014, 3:59 p.m..../Phototoxicity Not susceptible Susceptible Subcellular Imaging Improving Well-established High-throughput...effectiveness. Analyze Cell Signaling Pathways Known cellular signaling pathways can be investigated in a high-throughput... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....post-transcriptional gene expression across the majority of cellular pathways. Despite several years of research, the...pushed the field further with the creation of the largest miRNA sensor collection reported to date, containing... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m.... through my work on the audit committee. I’m particularly curious to see how that progresses in 2019 and...Before you can go off and do great things in a particular area or profession, you need to make sure you... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m.... Prevent Interruptions If you need to focus, a large set of headphones can act as a Do Not Disturb sign...would build a wall of pipette tip boxes when she particularly needed to focus, which was extremely effective... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m.... this process, you will first need to do some molecular cloning. Depending on whether you are trying to...send your plasmid to a company. For a few hundred dollars, they will inject the plasmid into fly embryos ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization during ligand detection... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....can adjust this by changing the voltage for the particular fluorophore prior to recording data. Increasing...you are looking at proteins that are expressed regularly in cells and might be upregulated or downregulated... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....light-based control of signaling pathways. This is particularly exciting because using light to control signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....Guide" from Nature Jobs Blog While Twitter is a popular platform for scientists, it’s not the only tool...statistics on what percent of scientists and engineers regularly use the platform, why scientists are using the... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....systems, methyltransferases, methylate DNA at particular sequences to prevent them from being degraded...Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....re happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So ...on many other labs that may be doing something similar. There may be other variants of this that are better...