We narrowed to 745 results for: nes
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TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....achieving knowledge. Or, as we in the storytelling business like to say, science is all about navigating conflict...
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In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....affinity for the ligand, especially if the modified amines are located near the ligand binding site. A selective... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m...., we hope we can help all researchers achieve happiness in their careers regardless of their end goals... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....track progress, adjust expectations, and record milestones in reaching goals. The more time and thought ... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....complicated (and again, expensive) the different machines are, and how many regulations are in place to ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....many of them focused more on social media or the phones hiding under their desks than listening to me. ... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....with a large range of characteristics (color, brightness, photostability, maturation, oligomerization)... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....lot of AAV particles are needed to get a few good ones into a cell to produce expression. The higher the... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....variant has an approximately 12-fold increase in brightness after ~100 action potentials. This is about twice... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....cells in the inner ear in a mouse model of human deafness (György et al., 2017), exo-AAV2 in mouse retina... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....Agreement (UBMTA) is one of the most commonly used ones for transfers between academic institutions. What... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....arrangement of the B-B’ and C-C’ palindromic sequences determines the orientation of the ITR, which can either ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....read and follow the journal’s specific author guidelines for reporting your materials. If you deposit ... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...