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We narrowed to 843 results for: NTS

Showing: 331 - 340 of 843 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...particular organelles and their contents, or perform in situ hybridization experiments. In many cases fluorescent...proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric...FP timers can be used in any situation where one wants to understand the relationship between the age of...find use in studies of animal development where events like the patterning of nascent tissues and the ... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...

  2. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...gRNAs targeting different sites of interest thus represents a straightforward means of exerting tight control...studying biosignature preservation in Mars analogue environments.     Alejandro (Alex) Chavez is a member of ...CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    .... AAV is usually the limiting reagent for my experiments, so I always make more AAV than I think I need...than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet. My ...higher if I used PEG precipitation, but for my experiments, I get enough virus from the cell pellet alone... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers before doing...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...

  4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...seriously damage public trust in scientists, experiments involving potential gene drives should be conducted...consequently use these precautions in their own experiments2,3. While that didn't work quite as well as anticipated4.... Ecological confinement involves performing experiments in a geographic area where escaped organisms ...be able to find mates2. For example, ongoing experiments attempting to build gene drives in tropical mosquito...flies6. It's also worth noting that gene drive experiments are less hazardous in organisms that seldom reproduce...multiplicative, and there are very few gene drive experiments that can't be performed with two or more confinement...

  5. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...balance my daily tasks, meet cross-team project commitments, respond to any issues raised by team members...across most, if not all, industries and work environments. This is why they are called transferable skills...ask about your creativity, your potential manager wants to hear a specific time that you thought outside...boss needs someone to step up, when a new venture presents itself even outside of work, consider challenging... know you possessed. Think about your shining moments. Recall a few times you really felt great about...If you are responsible for working with lots of students, managing inventories in the lab, or organizing...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...number of modules. Constraints on the order of the modules, because neighboring fragments require compatible...although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters... to combine the functions of different genetic elements to generate non-natural tools such as novel biological...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  7. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...performing these experiments. False positives can occur even in small scale experiments, although these...protein-protein interactions. In later years, improvements to yeast transformation protocols also helped... can either be generated using random genomic fragments from the organism of interest, or a defined cDNA...mating two single haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed...both false positive and false negatives, these experiments must be set up, conducted and analysed with care...

  8. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...base editing events in real-time but also enrich for base-edited cells in their experiments. In the past...TLDR: The eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA...reporter that detects and enriches for base editing events in vivo without relying solely on GFP (Katti et...adaptable tool to identify and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the Dow lab is...is a flexible reporter system for base-editing events in vivo. As GO relies on the correction of a mutated...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid detection however...presence of SARS-CoV-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the..., scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable...for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less primer in the pre-amplification...low-frequency cancer mutations from cell-free DNA fragments as well as health-related single nucleotide polymorphisms...using a dipstick in under one hour. While the lab points out that the protocol is not approved for clinical...

  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...codons encode single amino acids. Evolutionary constraints have molded which codons are used preferentially...in any organism. So what are the evolutionary constraints that lead to these preferences and what can we...2005). How does codon usage bias affect my experiments? While codon preferences can be very useful for...production of nonfunctional proteins and protein fragments respectively. Solving the problem of codon usage...your expression host before using them in your experiments. Expression of alternative tRNAs If you don’t...producing alternative tRNAs may not express sufficient amounts of your protein of interest. Regardless of which...
Showing: 331 - 340 of 843 results