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  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...leading to safer medicine and more successful experiments. It’s something that all scientists should understand...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...supervisory experience working with undergraduate students? On top of that, perhaps you were involved in ...officers working specifically to assist graduate students in job searching. Start your career planning early...

  4. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in...transducer will generate a numerical value which represents the amount of analyte present in the system at...

  5. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...consistent levels of Cas9 expression between experiments and over time (as compared to separate transductions...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ...

  11. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted.     ...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...

  13. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they...and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis goes head-to-head with...

  18. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

  20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review...
Showing: 661 - 680 of 838 results