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We narrowed to 872 results for: FER

Showing: 661 - 680 of 872 results
  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...host organism. Because transcription machinery differs between cell types or organisms, promoters must...eukaryotes and prokaryotes We have included two reference tables below listing some of the most common bacterial...Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...
  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...recent research @daria_gift_shch.     References and resources References Shemetov, A.A., Oliinyk, O.S. & ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...
  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...
  5. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...dice on a new gRNA in the future! References and Resources References Eva K. Brinkman, Tao Chen, Mario ... frame editing frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, ...
  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...the HIV-1 copy number, although the efficiency differed from 48-100% between strains. In a similar experiment...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...
  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost...turned down a faculty position because none of the offering institutes were able to provide the resources ...
  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...
  9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...of the program beneath it. You can jump to the different time stamps in the description to watch any sections...precisely. You can find a great discussion of different types of replication in Nosek and Errington 2017...scientific findings. This makes sense on many different levels - it’s hard to process large amounts of...
  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...irrespective of in silico prediction. These techniques differ in important ways, but can also complement one ...sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer to the clinic...https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target...
  11. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...Twitter feed will be all about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start... Jennifer Tsang...
  12. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...
  13. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...viral vector information. Each day, we’ll cover a different topic as shown in the graphic below. Follow the... Jennifer Tsang...
  14. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3, 2019 Hope...  Jennifer Tsang...
  15. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ... to 764 scientists at 375 organizations in 21 different countries. It's safe to say we keep our shipping...science support to our efficient and easy tech transfer process to our website and educational resources...
  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks...
  17. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...higher percentage of renewable sources. This program offers options of between 14% - 100% electricity supplied...have chosen to participate at the highest level offered. As a result, we will be drawing 100% of our electricity...
Showing: 661 - 680 of 872 results