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We narrowed to 895 results for: Ide

Showing: 661 - 680 of 895 results

  1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...DNA systems capable of storing data and computing inside living cells. He's also a co-founder of the BioBricks...Champion of Change, a program created as part of President Barack Obama's Winning the Future Initiative. ...proteins. That's central to everything that goes on inside a cell. Until we get a handle on precise, forward...promise that we could instead have things like peptide or RNA aptamers that act as genetically encoded...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...inclusion of some interesting case studies, and Zimmer provides several. In Part Three of the book, called simply... and her essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay...dismay of middle-aged scientists, the identification of Green Fluorescent Protein (GFP) and its development...(for funding, etc.) with that produced by the confident and flamboyant Watsons.” In Part Four, “New Science...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Microscopy. What is Expansion Microscopy?  ExM provides a straightforward answer to the space problem....minimized through tweaks to these steps. But even under ideal polymerization and digestion conditions, loss of...Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria ...Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide to optimization in X10 expansion microscopy. Nat...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...less potential to become resistant to? CRISPR may provide a method for doing just that. While challenges ...problem. Above all, we need new antimicrobials, ideally ones for which the development of resistance will... antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. ...CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...numbers, but the latter eliminates the step of identifying prey proteins after screening. It is also possible...activation. Including a prey-only control also provides a baseline level of reporter activation. Vary ... And most importantly: Independently validate identified binding partners with other techniques! Minimizing... While this may be difficult to resolve for a wide range of prey proteins, there are now a number of...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... defective gene, Xm, the sequence of which is identical to the correct gene, Xw, except for a single base...vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter... in a ‘GC’ sequence context. If this cannot be avoided, select the target AID version. Expect very low...PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using hybrid prokaryotic and vertebrate...

  7. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...Sinnamon et al., 2020). They introduced a mouse MECP2 guide RNA and a hyperactive version of the human RNA base...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...

  8. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...theory for plasmid incompatibility is due to an identification issue: the CBPs in the cell can’t tell the ... lost from the cell at a higher rate than the coresident plasmid. Asymmetric plasmid loss occurs for several...based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment will...Genomics of microbial plasmids: classification and identification based on replication and transfer systems and...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... Adamson Lab recently used a CRISPRi screen to identify additional host factors impacting prime editing...subthreshold voltage fluctuations, while having high fidelity during action potentials of fast-spiking neurons... combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia research...2023). Simple and Highly Specific Targeting of Resident Microglia with Adeno-Associated Virus. bioRxiv...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...pseudotyping allows these viruses to infect neurons. Besides altering the host tropism of your virus, there ... as a retrograde tracer so that scientists can identify the neurons that project to a given brain region... resources on the Addgene blog: Read the Quick Guide to All Things Lentivirus Get tips for titering your...Resources on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...growth phase are alive and healthy, which makes them ideal for protein expression. If you wait too long to ...and pLysE—the difference being that the latter provides tighter control of basal expression. What if I... growth media, or if your plasmid is low-copy, consider reducing the antibiotic concentration. Growth ...which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These...

  12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector, big transgene...of expressing a truncated protein. This can be avoided by excluding a stop codon from donor A in any of... to be reconstituted, there is currently no one ideal split vector system. Overall the efficiency of transgene...Resources on Addgene.org: Check out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... function — providing researchers new tools to identify integrin presence, study signaling mechanisms,...function-blocking integrin antibodies exist, almost all bind outside the ligand-binding pocket, their sheer size impeding...monoclonals, we anticipate that our chemically identical hybridoma-derived antibodies will also work in... More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... the thousands! The goal of this article is to provide enough background for you to distinguish the features...resistance to tetracycline Additionally, Table 2 provides a quick reference for some of the popular strains...strains are all based on E. coli K-12 and are considered BSL-1. Table 2: Lab strains of E. coli Strain...a Greek delta (Δ).  Additional resources We've provided an overview of the common lab strains; however...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...DNA-sequence configuration (state) based on the identity and order of a particular set of inputs, may be...sequencing.  A less straightforward, but more population-wide (as opposed to single-cell) approach is to interrogate...too expensive, then the qPCR method should be considered as an alternative. The qPCR method can be performed...researchers adapt GRSMs for their research, we provide MATLAB-based software that automates the design...

  16. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...updates whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared...bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what is...

  17. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...different? If you still need an extra push, there’s evidence that laughter really can be good for you. A search...laughter” turns up a few fun and very recent bits of evidence. (What can I say, we are scientists). Researchers...way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that we’ve convinced...

  18. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...cells that it can infect. The VSV-G envelope is widely used and requested because it enables lentiviruses...how lentiviruses work, check out our lentivirus guide page. For more protocols and information, be sure...requested plasmid of 2018! Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting...

  19. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ... with “A Better Way to Share Science” for: The inside scoop from scientists who have deposited plasmids...technician, or Principal Investigator. How-to guides for troubleshooting your experiments and getting...and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.  ...

  20. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...take samples from my assay at like 23:00, so I decided to make a giant snow angel for biologists in our...but to place it next to our lab trash (which coincidentally matches our Christmas tree's RGB colors) 😅...)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram by December...
Showing: 661 - 680 of 895 results