We narrowed to 711 results for: Met
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TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively...
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New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m.... of questions surrounding cancer development, metastasis, and drug resistance, Bhang says “the [drug] ... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....the plasmid to stop functioning properly or be something completely different than what was originally ... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....available destination vectors for various delivery methods, along with compatible inserts. All destination... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....al. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016. PubMed PMID: 27110581. PubMed Central... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....automate and optimize more protocols in the future - something that will be key for our future growth. One of... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m.... may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis,... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....chromosomes recombine during meiosis, creating gametes that contain a mix of genetic material from each... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting (FACS... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... does. Her parents told her that if she found something she loves to do, she would never work a day in... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....Krebs, A., Schübeler, D., Hajdú, R.I., Lukáts, Á., Németh, J., Nagy, Z.Z., Wu, K., Wu, R., Xiang, L., Fang... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....engineered. Fig. 1: Antibody production methods NeuroMab and others have an extensive library... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....the Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m.... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....allowing for rescued expression of GeneR. Sometimes also referred to as DiO (Double-floxed inverse... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....possible with conventional electrophysiological methods, it has remained difficult with GEVIs. Not only... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...