We narrowed to 699 results for: eed

1. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ... systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...transgene into a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB...

3. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice in bacteria. ...

4. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...promoter sequence. Thus, T7 RNA polymerase also needs to be present in the cells to transcribe the viral...

5. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...sounds a little neurotic, but hey- sometimes fun needs to be had in an orderly and timely fashion. 9. ...

6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...this versatile protein to suit their experimental needs, and some of the common applications in the lab....

7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ...arise and tools to deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish...

8. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components, ...

9. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...researchers can find the reagents and resources they need to cure Rett Syndrome.  If you’re new to Rett Syndrome...

10. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ... a protein in its native host there is likely no need to codon optimize as this gene should already be...

11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... for some applications, it doesn’t eliminate the need to deliver larger cargo such as base editors and...

12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...requirement for Cas9-mediated DNA cleavage. The need for more PAM sequences While PAM sequences for the...

13. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...sensitivity and specificity without compromising speed, ease of use, and portability. This method can be...

14. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...replication and the replication control machinery (Need a refresher on the Origin of Replication? See our...

15. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), ...

16. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ... (T) or cytosine (C). Several different teams succeeded with this approach around the same time (He et...

17. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...) containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a ...

18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...subsequent reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for several...

20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type

    Blog Post

    Published

    Nov. 15, 2017, 3 p.m.

    ... and thus are deemed reliable, or if the sample needs to be quantified again. Some of the issues with...