We narrowed to 682 results for: eng

1. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...

2. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

3. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...envelop protein. Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping...

4. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...alcohol dehydrogenase I Repressed by ethanol Full length version is strong with high expression. Truncated...

5. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...population can be seen as a stepwise decrease in signal strength in a flow plot. d = days post treatment. Created...

6. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold...

7. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...temptation to provide too many details. Reverse engineer your talk. Identify the key points you want to...

8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...priorities in mind, many researchers go to great lengths to keep accurate records, but given the complexities...

9. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...

10. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...2003): 8074–8075. PubMed PMID:12837056. 8. Pan, J. feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application...

11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity...

12. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...received a doctoral degree (or equivalent) and is engaged in a temporary and defined period of mentored advanced...

13. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ...success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental goals...

14. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ... Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG...

15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...goals from effecting our experiments. How do we engender better experimental design? The summation of many...

16. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...A stacking gel typically has a different ionic strength and lower pH and acrylamide content than the resolving...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ....2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An ...

18. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...lab Writes a lot of grants Varying degrees of engagement with what happens in the lab Official title is...

19. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...

20. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...requested through Addgene. For example, you could enter "Feng Zhang (Addgene plasmid #52961)".   The vector type...