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Showing: 661 - 680 of 769 results
  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...This workaround risks cellular phototoxicity and eliminates the possibility of long-term imaging studies....
  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... it is still bound to the glutathione resin, eliminating the need for an extra clean up step of the tag...
  3. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... with alternating spacers and repeats, was the original impetus for studying CRISPR. Spacers: Copies of...
  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining a llama facility...
  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... require high temperature reaction conditions. Finally, its longevity in human serum may allow for more...
  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...all out with constitutive high level expression? Finally, you can add bells and whistles to your construct...
  7. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...the research project must have innovative yet attainable milestones that contribute to intellectual thought...
  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...many aspects to choosing the right labs (see my webinar (updated link from 2020) on this topic), for this...
  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...
Showing: 661 - 680 of 769 results