We narrowed to 681 results for: mos

1. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ... plasmids all replicate in E. coli, which is the most commonly used bacteria in lab manipulations. Table...

2. An Inside Look into Addgene's Dev Team

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   Blog Post

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   Jan. 23, 2020, 2:45 p.m.

   ... third party libraries and any security updates. Most of the time, updating these libraries is straight...

3. CRISPR Activation: A Practical Guide

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   Blog Post

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   Aug. 18, 2016, 2:30 p.m.

   ...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...

4. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

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   Aug. 5, 2014, 6:51 p.m.

   ...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

5. Tips for Getting a Faculty Position

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   Blog Post

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   July 20, 2023, 1:15 p.m.

   ...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...

6. CRISPR 101: Validating Your Genome Edit

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   Blog Post

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   Nov. 3, 2022, 12:15 p.m.

   ... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

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   June 3, 2021, 1:15 p.m.

   ...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...

8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Blog Post

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   Feb. 22, 2017, 3:30 p.m.

   ...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...

9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

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   March 5, 2019, 1:08 p.m.

   ...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...

10. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

11. Site Directed Mutagenesis by PCR

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    Blog Post

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    Aug. 2, 2016, 2:30 p.m.

    ...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...

12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...

13. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...

14. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...

15. Plasmids 101: Restriction Cloning

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    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

16. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post

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    Aug. 5, 2025, 1:15 p.m.

    ...traction in recent years. The goal is to engineer the most miniscule parts of life, like proteins, to study...

17. Antibodies 101: Flow Cytometry

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    Blog Post

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    July 20, 2021, 1:15 p.m.

    ...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...

18. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...expressing Cre. The result of a system like this is a mosaic of cells labelled with different fluorescent proteins...

19. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...Rather than reprogram cells all the way back to their most primitive pluripotent stem cell state, through transdifferentiation...

20. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...photoactive Cas9 tool, as shown in the figure below. Their most successful design utilized Magnet photoswitchable...