We narrowed to 679 results for: mos

1. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...

2. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

3. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...

5. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...

6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...

7. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...

8. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

9. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...

10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...

11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...

12. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...

13. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    Published

    Aug. 5, 2025, 1:15 p.m.

    ...traction in recent years. The goal is to engineer the most miniscule parts of life, like proteins, to study...

15. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...

16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...expressing Cre. The result of a system like this is a mosaic of cells labelled with different fluorescent proteins...

17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...Rather than reprogram cells all the way back to their most primitive pluripotent stem cell state, through transdifferentiation...

18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...photoactive Cas9 tool, as shown in the figure below. Their most successful design utilized Magnet photoswitchable...

19. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...four adenine base editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency...