We narrowed to 855 results for: nts

...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...

...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ...

...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human...

...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...

...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated...

... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...

...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool....

...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...knowledge base to describe aptamers and SELEX experiments. Database, bas006 (2012). PubMed. For a stepwise...

...application is clinical; can we find ways to treat patients? Over a quarter of a million people have some ...activate a set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness...

...impact on the samples. We therefore devised a few experiments to determine if our tubes were permeable to the... the sample, which may have some effect on the contents of the sample. For example, one study found that...

...for pilot experiments and for interpreting the results of studies. When planning AAV experiments, scientists...

...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...

...temporal arrangement of chromosomes and how these arrangements may affect nuclear processes. The latest of ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...

...babies,” where fused genetic material from intended parents is “rehoused” in a separate, enucleated zygote ..., despite many headlines questioning if He’s experiments prove otherwise, the scientific process does ...

...feed. Due to increasing prices of methanol and advancements in other biomass production techniques in the...suited to it. Putting K. phaffii to work   The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere...

...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...leading to safer medicine and more successful experiments. It’s something that all scientists should understand...

...supervisory experience working with undergraduate students? On top of that, perhaps you were involved in ...officers working specifically to assist graduate students in job searching. Start your career planning early...

...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available...

... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ...