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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: LAS

Showing: 341 - 350 of 816 results

  1. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...fiberoptic cannulas directly implanted in the injection site and connected to a laser. The laser flashes light... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...received her PhD in Virology from the University of Glasgow. She then moved to France where she worked as a...

  2. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... scientists share plasmids across the globe, we’re working on ways to send plasmids as DNA as opposed ...fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered... transformation. With our ever growing rate of plasmid deposits (over 100 deposits per month!), manual...Qiagen BIOROBOT Universal Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, ...used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete this...There is a tube that goes from the needle to the flask with agar in it. The agar sits on a hot plate with...

  3. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...up your or your class’s streak plate skills? Find the chromoprotein expressing plasmids here, and check... Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at Addgene Check out additional...other species.  When he found the chromoprotein plasmids available from Addgene, he knew it would be a ...hopefully revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually...molecular biology. “What I'd like to do with the plasmids from Addgene is have the students transform them...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...for instance in inherited disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy...nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins where Adv vector delivery is often used...

  5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem. You're OK...tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech and is in a freezer...stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)A dimerizing protein...dramatic. For a membrane POI localized to the endoplasmic reticulum, the normally spiderweb-like pattern...dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing FP is fused...quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik...

  6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...2011). But this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely...Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic transition...two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP...fluorescent proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with ...researcher can flank fluorescent proteins in a plasmid with loxP sites such that their expression is turned... the brains of mice chronically infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009...cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog 5.7 (2009): e1000505. PubMed...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...cell membranes, so they are best used either with plasma membrane proteins (such as GPCRs, ion channels,...40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized cells  Cy5NTAc...38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ... the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ...its function. In nature Agrobacterium contains a plasmid with a T-DNA (transfer-DNA) region. During infection...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...computer. Eventually, our workflow would handle plasmid sequences on a 96-well plate basis, but for rapid...

  10. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    Published
    July 26, 2018, 12:46 p.m.
    ...suppressed and NES activity is enhanced, leading to cytoplasmic translocation of the reporter. As a result, the...phosphorylation status. The relative ratio of cytoplasmic vs. nuclear fluorescence serves as a proxy for...monitor global kinase activity that occurs in the cytoplasm and/or nucleus, while FRET-based reporters can...because KTRs shuttle between the nucleus and the cytoplasm, nuclear export and import rates can influence...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...
Showing: 341 - 350 of 816 results