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Addgene

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We narrowed to 599 results for: REV;

Showing: 341 - 350 of 599 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....

  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...antibody dilution — both factors we’ve covered in our previous blog posts (1, 2) on western blotting. If the ...confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...strategies. As an example, we’ll use one of my previous experiments where I harvested a melanoma tumor...single cell + live” population, acquired from the previous gating pipeline, about 53% of my cells are dendritic...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...your success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental...going through some of the questions we mentioned previously.  There is value in the process of creating a...

  8. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...means an exhaustive list and we encourage you to review the literature for your specific experimental technique...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA...repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    .... Of note, most of these optimized Cas enzymes prevent cutting at mismatched guide sites, but not necessarily...alternative pathways. See (Guo et al, 2023) for a review of other targeted sequencing methods. Candidate...
Showing: 341 - 350 of 599 results