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We narrowed to 848 results for: LEA

Showing: 681 - 700 of 848 results

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 ... Blog Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  12. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  14. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...step may not be necessary. In fact, you may get cleaner results by not permeabilizing as you’ll prevent...interacts with cholesterol in cell membranes and leaves many membrane-associated proteins in place. In ...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools suite, and...This process saves the QC team time, but does not leave much flexibility for discrepancies in backbone sequences...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  20. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...
Showing: 681 - 700 of 848 results