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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: LEA

Showing: 681 - 700 of 845 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...applications that don’t yet exist, but it wasn’t clear that this was even possible. There is a variety ...that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E. coli, in mammalian...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 ... Blog Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  9. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  10. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...step may not be necessary. In fact, you may get cleaner results by not permeabilizing as you’ll prevent...interacts with cholesterol in cell membranes and leaves many membrane-associated proteins in place. In ...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

  17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...containing glucose and L-arabinose at 37oC for at least 12 hours, or at lower temperatures for longer. Cultures... induction of expression… but longer expression leads to greater amount of gas vesicles and increased ...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools suite, and...This process saves the QC team time, but does not leave much flexibility for discrepancies in backbone sequences...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for. The result is redundancy...organism. So what are the evolutionary constraints that lead to these preferences and what can we do about them...
Showing: 681 - 700 of 845 results