We narrowed to 716 results for: Met

... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they...

...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

...near your insertion site. Base editing, another method to create point mutations, also has a strict editing...

... terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W...

...possible with conventional electrophysiological methods, it has remained difficult with GEVIs. Not only...

... inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

...have been continuously pursuing new and better methods to peer into the mysterious, microscopic world ...

... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic...

...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...

...the protein's behavior in cells, in solution or something in between, you are using a tag with a serious...

... citation for you to use in your Materials and Methods and References sections when citing the use of ...

... to include your improvements, or maybe even a methods paper. The problem with this is that it may take...

...responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably developed...

...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

... any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated...

...their plasmids are available from Addgene in the Methods & Materials or elsewhere in their papers. Maybe...

... screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed...

... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they...

...-target toxicity compared to other RNA editing methods — streamlining experimental design and its potential...

... associated with the library and reading their methods sections to see what worked.  Ready, optimize, ...