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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 849 results for: NTS

Showing: 681 - 700 of 849 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein...

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

  4. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they...and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis goes head-to-head with...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...-species staining protocols that use antibody fragments to block the endogenous immunoglobulins and reduce...complex with a variety of proteins interacting. Experiments tend to be equally complex with users trying ...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...

  16. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

  17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

  18. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... best strategies and protocols for different experiments.  In this blog post let's take a look at some...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...

  20. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...format depending on the particular style and requirements of the new Board Members. In some instances ...when it is so important for postdocs and graduate students to think about their career options, gaining transferable...
Showing: 681 - 700 of 849 results