We narrowed to 745 results for: nes
-
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m.... Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended to determine the turnover of ...
-
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....cloning strategy. Once you've identified potential clones after the screening, sequence the plasmid. Many... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....all-in-one acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....heavy chains. In the lab, this is the region that determines what secondary antibody you should use when performing... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m.... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore that are susceptible to redox... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....Some of these synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....how software can be structured to allow for the messiness of biology. These examples are intentionally trivial... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....limit of roughly 0.1%. Thus, rare, pre-existing clones that are present at less than 0.1% of the total... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m.... via plasmids or by the creation of stable cell lines and these cells are then grown in biotin-containing... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....scientists must genotype each animal to identify the ones with the desired genotype. Inducible and conditional... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....exceptions to our standard workflow. Table 1: Guidelines for the data fields in the deposit spreadsheet... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m.... 500 times the number of plaques than the linear ones. The 52-piece circular assembly of the T7 genome... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....S. (2019). DREADDs: The Power of the Lock, the Weakness of the Key. Favoring the Pursuit of Specific Conditions... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....maintaining the quality of reagents, especially shared ones. The importance of QC Plasmids are a fundamental...