We narrowed to 855 results for: nts

... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein...

...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in...transducer will generate a numerical value which represents the amount of analyte present in the system at...

... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must...

...diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available...

... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ...

...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted.     ...

...just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...

...and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they...and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis goes head-to-head with...

... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion...considering the use of pooled libraries in your experiments, Addgene recommends that you review the steps...

...genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review...

...control in many genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...

...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

...control spatially as to what’s happening at different points within the wood. There are different types of cellulose...hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting...

...under BSL1 conditions.  Patents or Licenses If there are any patents or licenses that could prohibit...

...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...

...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...