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TypeBlog PostPublishedNov. 8, 2016, 3:30 p.m....16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical cancer screening...Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank Seeding Labs for...
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Editor's Choice, October 2016
TypeBlog PostPublishedNov. 4, 2016, 2:30 p.m....podcast all about graduate student life and how establishing a thriving graduate student community can make...have tons of great advice for those of you just starting or pushing through your PhDs. Of course there ... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog PostPublishedSept. 1, 2016, 2:30 p.m....them difficult to approach for the youngster just starting in the lab, but they are exactly the people you...students are friendly and full of useful advice. Stay tuned for concrete advice on how to pick a lab and... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....bloggers each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m....mentorship and networking opportunities, publishing assistance, and more to Instrumental Access participants...Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the International Mentorship... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog PostPublishedFeb. 13, 2014, 1 p.m....can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and ...followed by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation... -
The Addgene Antibody Data Hub
TypeBlog PostPublishedSept. 12, 2024, 1:15 p.m.... your research, you've probably found yourself staring at a browser full of tabs, each open to a different...anti-Lhx6.1 antibody. For a more detailed understanding of the experimental conditions in which the ... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success. In conclusion, it is important for you to establish in advance how you want to utilize your plasmid... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m....protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....you're transforming a plasmid with ampicillin resistance. After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed. However, you can ... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started! I learned a lot about creative ways to do science... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....Design: FRET reporter activity depends on both the distance and the orientation of the donor and acceptor ...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m.... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon. Rest assured, as the... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m.... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....Twitter to find phages to treat an antibiotic resistant bacterial infection, she shared that tweet with...the search of phages for therapy Phage Directory started out as a directory of phage researchers and the... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m.... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...