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Showing: 351 - 360 of 602 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...individual plasmid orders, because when a PI or lab places an order, they are likely to order several plasmids...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers need...between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens ...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling so that a cell’s projections are easy to trace and not lost in a jumble of fluorescent neurons.... But notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green...

  8. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...samples accurately and in a way that can be easily traced. If you’re just starting a lab, or are looking ...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...
Showing: 351 - 360 of 602 results