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Showing: 701 - 720 of 838 results

  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...isotype controls are also commonly used in IF experiments. Precisely which controls you perform will depend...spectral properties match your available imaging instruments and the spectral properties do not overlap with...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...exposure. However, the limited availability of reagents and equipment and the long turnaround times, have...steps and the Cas protein used. Here, all the components needed for the reaction are mixed in a single...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase...

  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...that isolates proteins from different cellular compartments such as the mitochondria, cytoplasm and nucleus...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...digest appears normal and produces the expected fragments for that plasmid. This phenomenon indicates the...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors .... Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery ...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...you will find this information useful for your experiments. Don’t hesitate to check our lentiviral vector...

  13. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant...2018). Real‐time observation of flexible domain movements in CRISPR–Cas9. The EMBO Journal, 37(10), e96941...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...more genes, protein coding sequences, or other elements inserted into it. You will have the option to ...plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is a Bacterial...

  15. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...find an introduction to viral vectors and their elements; resources on different types of vectors, capsids...

  16. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...

  18. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like to give a shoutout to BosLab...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can we!...

  20. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...
Showing: 701 - 720 of 838 results