We narrowed to 803 results for: TIM

1. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   Published

   June 22, 2021, 1:15 p.m.

   ... B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization ...

2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m — Barbara Di Ventura...

3. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

   Type

   Blog Post

   Published

   May 25, 2021, 2:29 p.m.

   ...reagents and will enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been...

4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...eliminates the double-stranding step and reduces both the time and cost for AAV genome sequencing.  Figure...

5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   Published

   March 5, 2020, 2:15 p.m.

   ...titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection did you wait...

6. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...

7. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

   Type

   Blog Post

   Published

   Aug. 8, 2019, 1:19 p.m.

   ...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my...

8. New and Upcoming Viral Vectors - Spring 2019

   Type

   Blog Post

   Published

   March 26, 2019, 1:40 p.m.

   ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

9. Fluorescent Biosensors for Measuring Autophagic Flux

   Type

   Blog Post

   Published

   Jan. 22, 2019, 2:41 p.m.

   ...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...requires less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging...

11. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts. iTOP also...

12. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...not properly assemble, but these sequences can sometimes be removed to improve vector stability and sequencing...

13. Hot Plasmids - January 2023

    Type

    Blog Post

    Published

    Jan. 31, 2023, 2:15 p.m.

    ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...

14. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...these tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current ...

15. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    Published

    March 1, 2018, 2:11 p.m.

    ...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...

16. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    Published

    June 18, 2019, 1:15 p.m.

    ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

17. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    Published

    March 14, 2019, 1:54 p.m.

    ...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...

18. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...

19. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...teaching developmental biology Use the pathways over time plasmids to engage students in functional genomics...

20. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ...more specific procedural instructions, including timing, preparing the smear, and mixing the stains and...