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  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...actually from a completely unexpected finding in a different experiment, this tool was developed because a ...doing and sharing science in the first place?   References and Resources More resources on the Addgene blog...

  4. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...restriction enzymes that can both function in the same buffer, as this will save time later In our example,...Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the...

  6. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...translocations. It also provides researchers with many different ways to control gene expression. Get the full ...Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made...

  8. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

  9. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...but when it came time to add author details a different person in the lab wanted to be the first author...scientists in SciComm careers Check out our series on transferable skills ...

  10. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...rumors spread in our building that someone from a different company had tested positive for COVID-19 (it turned...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

  11. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...whether any given antibody is available through different companies (Bandrowski et al., 2016). It’s also... the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

  12. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

  13. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  14. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...

  15. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...time for exercise. Many studios and gyms are now offering online classes and participating in these is a... Jennifer Tsang...

  16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest...

  18. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  19. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...
Showing: 701 - 720 of 855 results