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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: LEA

Showing: 701 - 720 of 845 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...optimization within your experiment. Read on to learn more about the various controls that can be used...biological controls is independence: at the very least you should repeat your whole experiment from start...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the...plant species or cell type (Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9...

  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...(7). This modification allowed the deletion of release factor 1, the protein responsible for terminating...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression...

  8. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...governments exist in reality and science is how we learn about reality. Formally, a government can be defined...public discourse, many scientists have chosen to leave the lab and march. Unintended consequences While...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... 1). In general, the monomeric state of a probe leads to less interference with the oligomeric state of...indicators NIR-GECOs were developed based on mIFP6. Learn more about the characteristics, performance, and...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... in the classroom on our blog. Now that you’ve learned more about popular model organisms in the lab, ... about plasmids in our Plasmids 101 blog series Learn more about CRISPR and genome editing in our CRISPR...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...tip: When designing your PCR, ensure there is at least ~200 base pairs of sequence flanking the editing...find a null clone, you should screen, at the very least, 4 clones. Did you use a dual guide system to generate...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression of...expression. Co-expression of repressor QS along with QF leads to intermediate expression from QUAS, as seen with...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...used in this approach should also allow for the clear identification of the cell types of interest. By...Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A....

  19. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  20. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ... to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they...
Showing: 701 - 720 of 845 results