We narrowed to 855 results for: NTS

... best strategies and protocols for different experiments.  In this blog post let's take a look at some...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...

...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

...-species staining protocols that use antibody fragments to block the endogenous immunoglobulins and reduce...complex with a variety of proteins interacting. Experiments tend to be equally complex with users trying ...

...format depending on the particular style and requirements of the new Board Members. In some instances ...when it is so important for postdocs and graduate students to think about their career options, gaining transferable...

...when trying to perform high-throughput experiments. If a user wants to carry out a high-throughput genetic...

...heat- the inside of the wrist or the cheek. (Parents of infants will have no problem with this!) Make sure...

...essence, pluripotent cells can be thought of as descendants of totipotent cells. An example of pluripotent...that are produced during in vitro fertilization treatments and donated afterwards for ESC research purposes...

...the conference Review your favorite conference moments on social media and if there was something you ... with people you don’t know or attending social events. I like to set goals for myself like meet ten new...

...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...participating in development or other physiopathological events to which the chosen promoter responds. You can ...

...Because it is made at the same time as the other components of the vector particle, some of the RT protein... Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors...

...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...

...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally...for sequencing, representative NGS results (read counts or another visualization) that confirm diversity...

...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...

...tools that best fit their specific experimental requirements. Best-in-class collection at Addgene The GTA... . . . Levi, B. P. (2021). Functional enhancer elements drive subclass-selective expression from mouse...

...display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations compared...significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling prior to the...

... proteins in living cells and organisms. Both components of the chimera are encoded by DNA. Since researchers...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...

...exposure. However, the limited availability of reagents and equipment and the long turnaround times, have...steps and the Cas protein used. Here, all the components needed for the reaction are mixed in a single...