We narrowed to 813 results for: TIM

1. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...expression level, induce expression at an opportune time or go all out with constitutive high level expression...

2. 10 Ways to Share Your Science!

   Type

   Blog Post

   Published

   May 13, 2014, 8:34 p.m.

   ...necessarily bad news, but it is an argument for taking the time to hone your communication skills. So, where can...

3. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...fast and plates should be used witin 1 month for optimal efficiency. Beware of satellite colonies! Carbenicillin...

4. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...arms race" between phages and prokaryotes; the ultimate victor will be the species who has the strongest...

5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...eliminates the double-stranding step and reduces both the time and cost for AAV genome sequencing.  Figure...

6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   Published

   March 5, 2020, 2:15 p.m.

   ...titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection did you wait...

7. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...

8. Antibodies 101: Epitope Availability

   Type

   Blog Post

   Published

   Jan. 11, 2022, 2 p.m.

   ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...

9. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...variety of natural products. Recently, this group optimized their initial system by using a stronger promoter...

10. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ... B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization ...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m — Barbara Di Ventura...

12. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    Published

    May 25, 2021, 2:29 p.m.

    ...reagents and will enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been...

13. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my...

14. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    Published

    March 26, 2019, 1:40 p.m.

    ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

15. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    Published

    Jan. 22, 2019, 2:41 p.m.

    ...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

16. Viral Vectors 101: Transductions

    Type

    Blog Post

    Published

    April 30, 2024, 1:15 p.m.

    ...design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...

17. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ...

18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...requires less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging...

19. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts. iTOP also...

20. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...teaching developmental biology Use the pathways over time plasmids to engage students in functional genomics...