We narrowed to 816 results for: ache

1. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved genetically-encoded...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   Published

   May 21, 2020, 1:15 p.m.

   ...CsChrimson::tdTomato, an optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the ...

3. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

   Type

   Blog Post

   Published

   Feb. 16, 2021, 2:15 p.m.

   ...opportunities for promotion. It’s not uncommon for bachelor’s and master’s level scientists to have job titles...

4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

   Type

   Blog Post

   Published

   Sept. 12, 2023, 1:15 p.m.

   ...Additional resources were added in September 2023 by Rachel Leeson.  Additional resources on the Addgene ...

5. New Viral Vectors - Fall 2024

   Type

   Blog Post

   Published

   Oct. 3, 2024, 1:15 p.m.

   ...and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the...

6. Year of the Camelid: Antibody Style

   Type

   Blog Post

   Published

   May 9, 2024, 1:15 p.m.

   ... because maintaining a llama facility is out of reach and unappealing to many labs), the field is moving...

7. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...how should the gRNAs be oriented in comparison to each other? The gRNAs must target different strands to...

8. New CRISPR Web Resources and #12DaysofCRISPR Recap

   Type

   Blog Post

   Published

   Dec. 12, 2017, 3:13 p.m.

   ...sorting the plasmids we have available by category. Each plasmid page now has a helpful graphic to introduce...

9. Networking and Inspiring Young Scientists at Freshers Fairs

   Type

   Blog Post

   Published

   Nov. 17, 2015, 3:30 p.m.

   ...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes ...

10. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ...genotyping (using Sanger sequencing, for example) on each colony in order to screen for positive ones.  -...

11. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    Published

    Nov. 25, 2013, 10:12 p.m.

    ...339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. ...

12. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...constant regions. Variable regions are unique to each antibody and convey specificity to the target while...

13. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...

14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    Published

    March 5, 2020, 2:15 p.m.

    ...features like serotype, promoter, or tool type and each entry contains an image along with key experimental...

15. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ...While the various AAV serotypes can be used to achieve different patterns of expression, researchers often...

16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    Published

    Jan. 22, 2019, 2:41 p.m.

    ...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ...

17. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...addition GFP tags are large, dim, and permanently attached to a protein, making them hard to amplify and ...

18. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ...away from the loaded and judgemental language. In each peer-review, I try to mention what I liked about...

19. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    Published

    May 9, 2019, 1:15 p.m.

    ...acts as a unique identifier for individual cells. Each cell is labeled with 3-4 tags to increase the diversity...

20. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...Florida State University, they used a mutagenesis approach and found that specific amino acid changes favored...