We narrowed to 745 results for: nes

1. 6 Tips for Grant Writing

   Type

   Blog Post

   Published

   Oct. 18, 2016, 2:30 p.m.

   ...something that made them better than my unsuccessful ones. With grant applications there’s going to be an ...

2. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ... Abcam and NEB have Ph.D.s answering emails and phones). We can’t be experts on every technique or experiment...

3. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...orientation, and type of target sites that flox your genes of interest, a powerful FLEx switch can be created...

4. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...

5. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software produces quantifiable...

6. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera...

7. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...interview or campus visit in person, things like sickness or scheduling issues can and do interfere with...

8. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room for improvement...

9. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...

10. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems for genome engineering...

11. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ...the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a...

12. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...BioMolecular Modeling, we spun off a small, family-run business – 3D Molecular Designs – to commercialize some...

13. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

14. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ...science outreach: engaging the public to promote an awareness and understanding of science. Like most science...

15. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper...

18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction...

19. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...

20. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...