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We narrowed to 851 results for: nts

Showing: 701 - 720 of 851 results

  1. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...format depending on the particular style and requirements of the new Board Members. In some instances ...when it is so important for postdocs and graduate students to think about their career options, gaining transferable...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...the conference Review your favorite conference moments on social media and if there was something you ... with people you don’t know or attending social events. I like to set goals for myself like meet ten new...

  6. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...participating in development or other physiopathological events to which the chosen promoter responds. You can ...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein... Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

  9. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally...for sequencing, representative NGS results (read counts or another visualization) that confirm diversity...

  13. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...

  16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...exposure. However, the limited availability of reagents and equipment and the long turnaround times, have...steps and the Cas protein used. Here, all the components needed for the reaction are mixed in a single...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...isotype controls are also commonly used in IF experiments. Precisely which controls you perform will depend...spectral properties match your available imaging instruments and the spectral properties do not overlap with...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase ...needs to be taken into account when designing experiments.   {% module_block module "widget_0c4dbd65-f69b...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  20. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561...
Showing: 701 - 720 of 851 results