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TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...
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2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...practical guide by the workshop participants - a testament to openness and sharing in science. We’re excited... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....problem is especially stark in Drosophila, where expression of Cre from the standard UAS/GAL4 system is ... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m.... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....are located within their host is often key to understanding their role in both health and disease. To observe...constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research? arg1... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....become a popular model organism because their larval stage lends itself well to studies of neuroscience. The...escape behavior and spontaneous swimming. When startled by acoustic, visual or tactile stimuli, larval... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...the workspace. The color and size of icons are adjustable. New icons can be requested, although there’s... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....Optogenetic Tools Like pDawn Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... order to manage insect pests and to support sustainable agriculture. For this project the expression ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here! Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...