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  TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...
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  Celebrating Accomplishments in the LabTypeBlog PostPublishedMay 8, 2014, 3:07 p.m.... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...
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  Tag Your Favorite Yeast Genes with EaseTypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...
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  Three Key Considerations for Precise Neuronal Targeting Using AAV TechnologiesTypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...practical guide by the workshop participants - a testament to openness and sharing in science. We’re excited...
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  Working 9-5: an Addgene Co-opTypeBlog PostPublishedJune 2, 2022, 2:15 p.m....back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover...
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  2023 and 2024: Reflections and Looking AheadTypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating...
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  Increasing Your Work's Visibility with Addgene: Citation, Search, and CollectionsTypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ...
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  Cre-ating New Methods for Site-specific Recombination in DrosophilaTypeBlog PostPublishedMay 12, 2015, 1:32 p.m....problem is especially stark in Drosophila, where expression of Cre from the standard UAS/GAL4 system is ...
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  Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter GenesTypeBlog PostPublishedJune 19, 2018, 1:38 p.m....are located within their host is often key to understanding their role in both health and disease. To observe...constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research? arg1...
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  Zebrafish as a Model for Behavior: Swimming into the Optogenetic SpotlightTypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....become a popular model organism because their larval stage lends itself well to studies of neuroscience. The...escape behavior and spontaneous swimming. When startled by acoustic, visual or tactile stimuli, larval...
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  Early Career Researcher Toolbox: Free Tools for Making Scientific GraphicsTypeBlog PostPublishedJune 11, 2019, 1:16 p.m....When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...the workspace. The color and size of icons are adjustable. New icons can be requested, although there’s...
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  Chemogenetics vs. Optogenetics: Which Method Should I Choose?TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...
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  Hot Plasmids - October 2022TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here! Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...
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  CASTing Off for New Shores in Human Genome EditingTypeBlog PostPublishedJune 10, 2025, 1:15 p.m.... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...
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  Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....Optogenetic Tools Like pDawn Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... order to manage insect pests and to support sustainable agriculture. For this project the expression ...
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  SunTag and Fluorescent ImagingTypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ...
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  Embracing Serendipity: A Crucial Element in the PhD JourneyTypeBlog PostPublishedApril 25, 2024, 1:15 p.m....open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and...
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  Addgene at the ASM Microbe 2016 ConferenceTypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see...
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  Walking beside Academia and giving it a high five: My transition from graduate work to a position at AddgeneTypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while...
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  Choosing Your Perfect Empty BackboneTypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close...