We narrowed to 842 results for: NTS
-
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....offer two simple steps to welcome these unforeseen moments. Widening your search The first step towards inviting...breakthroughs often happen when we allow ourselves moments of release and mental freedom. Practical approaches...embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It's beneficial ...fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling in the... contemplate our approach to life and how our viewpoints influence our encounters. It underscores the ...
-
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....digestion, fragments are released from nuclei by a short incubation at 37 °C. These short DNA fragments can ...differential access to transcriptional regulatory elements is controlled during development using the Drosophila... preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using it in your own...Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, the most significant... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....Reading and exploration including replications and experiments resulting in “negative data” often dominate the...For instance, long before a study with multiple patients was published, Zika researcher Christa Osuna collated... interim research products (pre-registration, preprints, data, software, micropublications) in grant proposals...you the hard work of reading papers and doing experiments but we can definitely give you credit for it ...Addgene Blog Use Apps to Save Time and Track Your Experiments Use video to share your science at We Share Science... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m.... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...fluoresces when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit...peaks of DA release. Using dLight1 sensors in experiments The Tian lab used dLight1 both in brain slices...receptors, respectively). Using GRABDA sensors in experiments The Li lab used GRABDA in drosophila, zebrafish...here! Overall these sensors are powerful new developments in the detection and measurement of neurotransmitters... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders in the field of ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over... on that week’s topic by the moderators. The assignments, which consisted of reading that class’s section... wordsmith. But first there was an assignment. Students coming to the writing workshop in D.C. had to ...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS. Additional Resources on the Addgene Blog... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS... known to trigger seed germination of parasitic plants, this approach was also used to build and validate...Qi's lab, allows simultaneous gene activation in plants. FnCas12a, unlike other Cas12a nucleases identified...in moderate thermophiles. These evolved SpCas9 variants have improved activity on NAG PAMS and reduced...sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....They provide accounts of their research struggles in papers. They recount accomplishments in talks and... pay attention. Highlight your lowest points and highest points and make clear what was difficult and ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color...therefore can be used in immunoprecipitation experiments with other fluorescent proteins. Find mNeonGreen... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved in ...weeks and with labs worldwide closing for unknown amounts of time, we were facing a financial crisis. As ... resource for scientists that helps them share reagents — leading to open, reproducible science,” said...More scientists are sharing plasmids and other reagents every day, and this crisis has highlighted the... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m.... about Addgene. “The mission to share critical reagents such as plasmids and viral stocks for researchers...otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier...targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology...see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...