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  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...through the stomata into the abaxial site (lower surface) of the leaf. This is a simple and easy technique...Mortensen et al., 2019): Leaves or whole plants are placed in an Agrobacterium solution and a vacuum is applied...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...struggling to understand how the researchers decided to place their gates on the plots! Flow data is messy, so...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...are 27 incompatibility (Inc) groups in the Enterobacteriaceae family alone, and this is expanding as we...expelled antibiotic resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and vary in shape and structure. Their porous surface provides a large area for binding, but antibodies... than agarose beads. Proteins conjugate on the surface of magnetic beads, and are therefore completely...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...commercial antibodies can be tethered on the viral surface, expanding the range of targetable cell types. ...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...without disrupting plasmid representation. A good place to start is with a protocol for a library of similar...Moderated by the Feng Zhang lab, this is a useful place to ask experimental questions.  Practical Considerations...

  10. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... or the statistical variation is calculated and placed in the legend. Widely reported difficulties in ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate...
Showing: 361 - 370 of 602 results