We narrowed to 803 results for: TIM

1. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...

2. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...

3. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...

4. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   Published

   Dec. 18, 2014, 4:06 p.m.

   ...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

6. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   Published

   March 25, 2014, 3:15 p.m.

   ...the foreign DNA will be degraded or diluted over time. This, however, may not be a problem depending on...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...harness the natural diversity of CRISPR systems, only time will tell how large the genome and transcriptome...

9. Viral Vectors 101: Viral Vector Elements

   Type

   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...

10. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends ...

11. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...simultaneously in your system instead of just one at a time. Many of these libraries are packaged and delivered...

12. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

13. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...in the areas in which they have expertise. Other times, we may need to reach out to an external expert ...

14. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ...directions. I would like to thank them for their time, dedication, advice and encouragement. Why virus...

15. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

16. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...important to remember that it’s not the amount of time you spend on social media, but how you use social...

17. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...encounters  an antigen for a second or subsequent time. It is expressed as a monomer with a valency of ...

18. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ... may be undesirable in many applications. At the time of our first blog post on choosing a Cas9, the Feng...

19. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied...

20. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...interactions between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF...