We narrowed to 801 results for: Des

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

...retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...

...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...

...of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents...

...Define the scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab ...

...University of British Columbia, Student Services - includes videos on general resume writing, as well as additional...

...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ...

...different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the...

...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...