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TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...
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Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....producer cells and purification protocols often describe a process that begins with cell lysis to release... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....shipment processes. After reading a publication describing how short term storage on dry ice can shift sample... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....Define the scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab ... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....University of British Columbia, Student Services - includes videos on general resume writing, as well as additional... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV.... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m.... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m.... from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs)...