We narrowed to 872 results for: FER

...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

...but when it came time to add author details a different person in the lab wanted to be the first author...scientists in SciComm careers Check out our series on transferable skills ...

...the issue is not unique to IHC.  References and Resources References Goodpaster T, Randolph-Habecker J...

...rumors spread in our building that someone from a different company had tested positive for COVID-19 (it turned...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

...whether any given antibody is available through different companies (Bandrowski et al., 2016). It’s also... the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

...actually from a completely unexpected finding in a different experiment, this tool was developed because a ...doing and sharing science in the first place?   References and Resources More resources on the Addgene blog...

...restriction enzymes that can both function in the same buffer, as this will save time later In our example,...Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the...

...translocations. It also provides researchers with many different ways to control gene expression. Get the full ...Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made...

...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...

...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...

...time for exercise. Many studios and gyms are now offering online classes and participating in these is a... Jennifer Tsang...

...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest...

...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...

... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...

...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ...