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  1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer...

  3. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ...

  5. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...

  9. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  12. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

  14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ...

  18. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... to address the unique needs of students from different departments across the university and alterations...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

  19. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...-op. It has been so insightful to learn about different career paths, successes and failures, values, ...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...
Showing: 721 - 740 of 855 results