We narrowed to 777 results for: INA

1. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   .... (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two...

3. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ...energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his...

5. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...feedback for improvement. Go to a science or career seminar together and meet to debrief on the topic (career...

6. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ... you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for...

7. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...could meet your needs.    Multiplex experiments Finally, consider whether you will be using other antibodies...

8. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...

9. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety ...

10. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...antibodies are produced in animals instead of via recombinant DNA, they can’t be genetically tagged and instead...

11. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...Hutch, so she attends that division’s scientific seminars a couple days a week. And there there’s always...

12. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...comfortably engage with you. If you can give a seminar, lead a journal club, or present at lab meetings...

13. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require...

14. All in a Twist: dsRNA

    Type

    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ... allowing the DNA strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle...

15. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...

16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    .... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

17. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization...

19. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for...

20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...conformationally restricted RNA which improves mismatch discrimination and is more nuclease-resistant, resulting in...