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We narrowed to 895 results for: Ide

Showing: 721 - 740 of 895 results

  1. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address... desired titer and want to dilute it, err on the side of caution when adding buffer back to the solution... for Work with Virus Watch Our AAV Purification Video ...

  4. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields...interact and learn more about one another. They also provided both breakfast, lunch, and snacks. At the end ... to constantly update our processes and get new ideas from learning experiences like workshops at Lab ...

  5. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...is very plausible that science published in more widely-read journals gets more citations and is more likely...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  6. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with...the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-CoV-2 (...You could be the next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected...

  7. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome...applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent...CRISPR/Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...

  9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD, the researchers...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you ...

  10. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...disease models established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  11. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...used to infer differentiation trajectories and identify both up- and down-regulated pathways. Individual...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity... a model system. TF screens can be designed to identify custom TF programs to acutely initiate disease...

  12. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...bioscience and tech transfer communities who help guide and promote Addgene in its mission to make research...given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench...a tremendous amount of technology development alongside it.” Doench starting working with David Root ...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...proteins to be imaged together, you will need to consider the same factors as when choosing an individual... the filter sets or lasers on your microscope. Ideally, you would like to have substantial overlap between...protein and a filter set, many filter set vendors provide tools to plot the fluorescence spectra of proteins...

  14. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...detection of the reporter in a specific assay, which ideally would have low background signal, high sensitivity...bioluminescent reporter, other luciferases have been identified in various species with differing kinetics, substrate...luciferase used, a compatible substrate must be provided for the enzyme to catalyze the light-producing...

  16. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often these methods...Institute Target Accelerator Plasmid Collections, now widely available to the scientific community through Addgene...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

  17. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a brief comparison...Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell...Research Catch up on Your CRISPR Background with Our Guide Pages ...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...there are many great resources available to help guide you through the rough waters of mutation validation...great deal of precision. This means digital PCR provides an absolute measure of target abundance, does ...screening to validate your genome edits, this paper provides detailed instructions on how to design such assays...

  19. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... kB, then you would be oversized even before considering the need for some manner of polyA. In this scenario...can be safely concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier...Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal... and have appropriate factors displayed on the outside of the particle to interact with the host. This...applications and aid in ease of use. Our lentiviral guide outlines these components.     Fig. 1 - ...
Showing: 721 - 740 of 895 results