We narrowed to 813 results for: TIM

1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   Published

   June 23, 2022, 1:15 p.m.

   ...scientists are using sgRNA for selection and optimization. In addition, the current work in developing...

2. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   Published

   June 18, 2019, 1:15 p.m.

   ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

3. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...

4. Creating accessible biology activities in schools with BioBits

   Type

   Blog Post

   Published

   Aug. 2, 2018, 12:56 p.m.

   ...teaching developmental biology Use the pathways over time plasmids to engage students in functional genomics...

5. FPbase: A new community-editable fluorescent protein database

   Type

   Blog Post

   Published

   May 16, 2018, 1 p.m.

   ...these tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current ...

6. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   Published

   March 1, 2018, 2:11 p.m.

   ...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...

7. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...

8. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...

9. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...

10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type

    Blog Post

    Published

    April 14, 2016, 2:30 p.m.

    ...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...

11. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

13. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    Published

    March 25, 2014, 3:15 p.m.

    ...the foreign DNA will be degraded or diluted over time. This, however, may not be a problem depending on...

14. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...in the areas in which they have expertise. Other times, we may need to reach out to an external expert ...

15. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied...

16. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...encounters  an antigen for a second or subsequent time. It is expressed as a monomer with a valency of ...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ... may be undesirable in many applications. At the time of our first blog post on choosing a Cas9, the Feng...

18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

19. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...important to remember that it’s not the amount of time you spend on social media, but how you use social...

20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...harness the natural diversity of CRISPR systems, only time will tell how large the genome and transcriptome...