...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...

...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success...

...incredibly useful, yet simple system allows you to systematically study gene function with temporal control and...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the coordinators...

...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols...

...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...

... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...

...other proteins that require post-translational modification may not be easily expressed in non-mammalian...are intended for the high level expression and purification of SARS-CoV-2 proteins for downstream experiments...and we’re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids and Resources...

... specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...PI labs and institutions. There are many more technical details that make our new search engine unique...

...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...

... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna...

... the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...directed evolution, because channelrhodopsins are typically evaluated with low-throughput methods such as ... midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light...

...s outer membrane and allows the components to physically enter the cells so they can go to work. ...Allen Cell Explorer, where you can also find the certificate of analysis provided with each cell line. The...

...using either loxP or lox511 sites, leaving two identical sites on one end of the DNA fragment (Figure 1...recombination event then excises the DNA between the identical loxP or lox511 sites (Figure 1, bottom). Since...presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead of BFP....

...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...